Perturbation of m6AWriters Reveals Two Distinct Classes of mRNA Methylation at Internal and 50 Sites

Schraga Schwartz,1,12 Maxwell R. Mumbach,1,12 Marko Jovanovic,1 Tim Wang,1,2,3 Karolina Maciag,1,4 G. Guy Bushkin,2 PhilippMertins,1 Dmitry Ter-Ovanesyan,1 Naomi Habib,1 Davide Cacchiarelli,1,5 Neville E. Sanjana,1 Elizaveta Freinkman,2 Michael E. Pacold,2,6 Rahul Satija,1 Tarjei S. Mikkelsen,1,5 Nir Hacohen,1,7 Feng Zhang,1,8,9 Steven A. Carr,1 Eric S. Lander,1,3,10,* and Aviv Regev1,3,11,* 1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA 2Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA 3Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA 4Graduate Program in Immunology, Division of Medical Sciences, Harvard Medical School, Boston, MA 02115, USA 5Harvard Stem Cell Institute and Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA 6Department of Radiation Oncology, Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA 02215, USA 7Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Charlestown, MA 02129, USA 8McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA 9Department of Brain and Cognitive Sciences and Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA 10Department of Systems Biology, Harvard Medical School, Boston, MA 02114, USA 11Howard Hughes Medical Institute, 4000 Jones Bridge Road, Chevy Chase, MD 20815, USA 12Co-first author *Correspondence: [email protected] (E.S.L.), [email protected] (A.R.) http://dx.doi.org/10.1016/j.celrep.2014.05.048 This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).